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Protein Biochemistry
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BCA Protein Assay Kit
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BCA Protein Assay Kit
BCA Protein Assay Kit
BCA Protein Assay Kit from Cell Signaling Technology
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Citations:
(42)
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BCA Protein Assay Kit
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BCA Protein Assay Kit
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Cell Signaling Technology
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7780S
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1 Kit
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (42)
(1)
Hawthorn leaves flavonoids attenuate cardiac remodeling induced by simulated microgravity
Pharm Biol
December 1, 2023
Liu T, Ma Y, Zhao H, Wang P, Niu Y, Hu Y, Shen X, Zhang M, Yan B, Yu J.
was centrifuged at 12000 g for 20 min at 4 °C. Protein concentration was determined using a BCA kit (
7780S
,
Cell Signalling
Technology, MA, USA) and adjusted with loading buffer (P0015A, Beyotime, Shanghai,
(2)
Pooled endogenous protein tagging and recruitment for scalable discovery of effectors for induced proximity therapeutics
Research Square
October 18, 2023
Yevgeniy V. Serebrenik, Yevgeniy Serebrenik, Timothé Maujean, George M. Maujean, George Burslem
(MilliporeSigma, P8340). Samples were normalized by bicinchoninic acid (BCA)
assay
(
Cell Signaling
,
7780
), and loaded on a precast
SDS-PAGE gel
(
Bio-Rad
,
4561086
).
Western blotting
followed using standard
(3)
Human Placental LRP5 and Sclerostin are Increased in Gestational Diabetes Mellitus Pregnancies
The Journal of clinical endocrinology and metabolism
September 18, 2023
Papadopoulou A, Thymara E, Maratou E, Kanellopoulos G, Papaevangelou V, Kalantaridou S, Kanellakis S, Triantafyllidou P, Valsamakis G, Mastorakos G.
Cell Signaling
USA). The concentration of the proteins was determined using the
BCA Protein Assay Kit
(#
7780S
,
Cell Signaling
, USA). Then,
Western blotting
for expression levels of the
LRP5
and of β-actin, used
(4)
mTOR Regulation of N-Myc Downstream Regulated 1 (NDRG1) Phosphorylation in Clear Cell Renal Cell Carcinoma
International Journal of Molecular Sciences
May 27, 2023
Anisha Valluri, Jessica Wellman, Chelsea L. McCallister, Kathleen C. Brown, Logan Lawrence, Rebecca Russell, James Jensen, James Denvir, Monica A. Valentovic, Krista L. Denning,
et al.
Protein concentration in lysate was measured with a
BCA protein assay kit
(
Cell Signaling Technology
,
7780
). Protein lysate (5 to 15 μg) was diluted in 2x laemmli sample buffer with β-mercaptoethanol, heat denatured
(5)
METTL14 Regulates PLAGL2/β-Catenin Signaling Axis to Promote the Development of Nonsmall Cell Lung Cancer
Journal of oncology
January 1, 2023
Zhou Q, Lai X, Gao Y, Chen Q, Xu Y, Liu Y.
total protein from cells and tissues. Proteins were quantified according to the
BCA protein assay kit
(#
7780
,
Cell Signaling Technology
, USA) and isolated by 10% SDS-PAGE
electrophoresis
. Electrotransfer transferred
(6)
The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity
iScience
July 15, 2022
Feng Chen, Runliu Wu, Jiao Liu, Rui Kang, Jinbao Li, Daolin Tang
al., 2010). Concentrations of proteins in the supernatant were determined by a
BCA Protein Assay Kit
(
7780
,
Cell Signaling Technology
). Prior to
immunoprecipitation
, samples containing equal amounts of proteins
(7)
Pristimerin inhibits neuronal inflammation and protects cognitive function in mice with sepsis-induced brain injuries by regulating PI3K/Akt signalling
Pharm Biol
December 1, 2021
Xue W, Li Y, Zhang M.
China); and
PI3K
(
4292
,
Cell Signaling Technology
, Shanghai, China). A bicinchoninic acid
assay
kit (
7780
,
Cell Signaling Technology
, Shanghai, China) was used to
measure
protein concentrations prior to
gel electrophoresis
(8)
Melatonin inhibits proliferation, migration, and invasion by inducing ROS-mediated apoptosis via suppression of the PI3K/Akt/mTOR signaling pathway in gallbladder cancer cells
Aging (Albany NY)
September 30, 2021
Kunlun Chen, Pengfei Zhu, Wenhui Chen, Kai Luo, Xiao-Jing Shi, Wenlong Zhai
denaturation at 100°C for 10 min, concentrations of proteins were assessed with the
BCA Protein Assay Kit
(#
7780
,
Cell Signaling Technology
Inc, CST, MA, USA) as described [21]. The equal total protein (30 μg) amounts
(9)
In vivo inducible reverse genetics in patients' tumors to identify individual therapeutic targets
Nature communications
September 27, 2021
Carlet M, Völse K, Vergalli J, Becker M, Herold T, Arner A, Senft D, Jurinovic V, Liu WH, Gao Y,
et al
.
Cell Signaling Technology
, Boston, USA) on ice for 30 min and protein concentration measured by BCA
assay
(#
7780
, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple).
(10)
A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
Scientific reports
June 16, 2021
Liu WH, Völse K, Senft D, Jeremias I.
Boston, USA) and
PMSF
(
1:200
) on ice for 30 min. Protein concentration was measured by BCA
assay
(#
7780
, New England Biolabs, Beverly, USA) and abundance of specific proteins determined by capillary
immunoassay
.
(11)
MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7
Folia Neuropathologica
May 10, 2021
Xiaojie Li, Lihe Yuan, Jin Wang, Zun Zhang, Shaojing Fu, Shaobin Wang, Xinhui Li
Danvers, MA, USA). Protein concentration was determined with a Bicinchoninic
protein assay
(BCA) kit (
7780S
,
Cell Signaling Technology
, Danvers, MA, USA). All the protein samples were then electrophoresed via
(12)
Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6-p-STAT3-p63-JAG2 pathway
Stem cell research & therapy
March 29, 2021
Peng W, Chang M, Wu Y, Zhu W, Tong L, Zhang G, Wang Q, Liu J, Zhu X, Cheng T,
et al
.
supernatants were used to
measure
total protein levels with the Bicinchoninic Acid (BCA) Protein Assay Kit (
7780
,
Cell Signaling Technology
[CST], Boston, MA, USA). Myeloperoxidase (MPO) activity determination The
(13)
Inhibition of the mevalonate pathway improves myocardial fibrosis
Experimental and Therapeutic Medicine
January 18, 2021
Huifeng Xu, Yi Shen, Chenyu Liang, Haifeng Wang, Junling Huang, Pengcheng Xue, Ming Luo
protein extraction
and protein concentration was determined using bicinchoninic acid
protein assay
reagent (cat. no.
7780
;
Cell Signaling
EXPERIMENTAL AND THERAPEUTIC MEDICINE 21: 224, 2021 3 Table I. Primer sequences
(14)
Activity of the growth hormone-releasing hormone antagonist MIA602 and its underlying mechanisms of action in sarcoidosis-like granuloma
Clinical & translational immunology
January 1, 2021
Zhang C, Tian R, Dreifus EM, Hashemi Shahraki A, Holt G, Cai R, Griswold A, Bejarano P, Jackson R, V Schally A,
et al
.
supernatant was collected. Protein concentration was determined by the
BCA protein assay kit
(Cat #
7780
;
Cell Signaling Technology
). Thirty micrograms of total protein was mixed in a reducing sample buffer
(15)
GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR Signaling
International journal of chronic obstructive pulmonary disease
January 1, 2021
Peng W, Wu Y, Zhang G, Zhu W, Chang M, Rouzi A, Jiang W, Tong L, Wang Q, Liu J,
et al
.
Waltham, MA, USA). Total protein levels in BALF were measured by Bicinchoninic Acid Protein Assay Kit (#
7780
,
Cell Signaling Technology
, Boston, MA, USA) as per the manufacturer’s protocol. IL-6, IL-1β, and
PLAU
(16)
Long Noncoding RNA CTC Inhibits Proliferation and Invasion by Targeting miR-146 to Regulate KIT in Papillary Thyroid Carcinoma
Scientific Reports
December 1, 2020
Baochun Liao, Shi Liu, Jiafeng Liu, Pulusu Ajay Kumar Reddy, Yong Ying, Yang Xie, Jianhua Wang, Xiangtai Zeng
Technology, 9800). Protein concentrations were quantified using BCA
assays
(
Cell Signaling Technology
,
7780
). Cell lysates (40 μg) were electrophoresed using 12%
SDS-PAGE
, and were then transferred to a nitrocellulose
(17)
Aberrant methylation of GADD45A is associated with decreased radiosensitivity in cervical cancer through the PI3K/AKT signaling pathway
Oncology Letters
November 3, 2020
Meng Lou, Rong Li, Ting-Yuan Lang, Li-Ying Zhang, Qi Zhou, Li Li
subsequent experimentation. Protein concentrations were determined using a
BCA Protein assay kit
(cat. no.
7780
,
Cell Signaling Technology
, Inc.) and ~40 µg protein/lane was separated via
SDS‑PAGE
on a 10% gel at
(18)
Extracellular ubiquitin promotes hepatoma metastasis by mediating M2 macrophage polarization via the activation of the CXCR4/ERK signaling pathway
Annals of Translational Medicine
September 21, 2020
Jiajing Cai et al.
(Beverly, MA, USA). MMP-9 (#76003), iNOS (#178945), TGF-β (#179695), CXCR4 (#181020/124824) and Ub (#
7780
) were obtained from Abcam (Cambridge, MA, USA). Animals Male C57BL/6
mice
aged 6 to 8 weeks were purchased
(19)
miR-125a-5p inhibits glycolysis by targeting hexokinase-II to improve pulmonary arterial hypertension
Aging (Albany NY)
May 19, 2020
Li Luo, Lusheng Xiao, Guili Lian, Huajun Wang, Liangdi Xie
(20)
Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/β-catenin
Stem cell research & therapy
May 19, 2020
Yang X, Ma X, Don O, Song Y, Chen X, Liu J, Qu J, Feng Y.
from differently treated MSCs. The protein concentration was determined using the BCA
assay
(
CST
, #
7780
, USA). Proteins were separated on
SDS-PAGE gel
ahead of being transferred on PVDF
membranes
(Life Technologies,
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BCA Protein Assay Kit from Cell Signaling Technology
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